| Autor: |
Jessica, Pandohee, Brendan J, Holland, Bingshan, Li, Takuya, Tsuzuki, Paul G, Stevenson, Neil W, Barnett, James R, Pearson, Oliver A H, Jones, Xavier A, Conlan |
| Rok vydání: |
2015 |
| Předmět: |
|
| Zdroj: |
Journal of separation science. 38(12) |
| ISSN: |
1615-9314 |
| Popis: |
Widely known for its recreational use, the cannabis plant also has the potential to act as an antibacterial agent in the medicinal field. The analysis of cannabis plants/products in both pharmacological and forensic studies often requires the separation of compounds of interest and/or accurate identification of the whole cannabinoid profile. In order to provide a complete separation and detection of cannabinoids, a new two-dimensional liquid chromatography method has been developed using acidic potassium permanganate chemiluminescence detection, which has been shown to be selective for cannabinoids. This was carried out using a Luna 100 Å CN column and a Poroshell 120 EC-C18 column in the first and second dimensions, respectively. The method has utilized a large amount of the available separation space with a spreading angle of 48.4° and a correlation of 0.66 allowing the determination of more than 120 constituents and mass spectral identification of ten cannabinoids in a single analytical run. The method has the potential to improve research involved in the characterization of sensitive, complex matrices. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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