| Autor: |
Alexandra, Hudecova, Katarina, Hasplova, Eva, Miadokova, Zuzana, Magdolenova, Alessandra, Rinna, Eliska, Galova, Andrea, Sevcovicova, Dagmar, Vaculcikova, Fridrich, Gregan, Maria, Dusinska |
| Rok vydání: |
2010 |
| Předmět: |
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| Zdroj: |
Neuro endocrinology letters. 31 |
| ISSN: |
0172-780X |
| Popis: |
The purpose of this study was to assess whether a methanol extract isolated from the flower of Gentiana asclepiadea had potential cytotoxic or genotoxic effect on COS 1 (monkey kidney) cell line. Five various concentrations of the extract were investigated for cytotoxicity and genotoxicity and to determine non-cytotoxic and non-genotoxic concentrations suitable for utilization in pharmacology and medicine.Cytotoxicity was determined using the proliferation (growth activity) and the plating efficiency (colony forming ability) assays after 24 hour incubation of COS 1 cells with different concentrations of methanolic flower extract from G. asclepiadea. To assess potential genotoxicity, the comet assay or SCGE (Single-Cell Gel Electrophoresis) was used.We found that only the highest (5 and 25 mg/ml) concentrations of the extract revealed cytotoxic and genotoxic effect. We have also determined concentrations that stimulated cell growth (0.25 mg/ml) and colony forming ability (0.25-2.5 mg/ml) and did not exhibit genotoxic effect (0.25-2.5 mg/ml).We found out that extract of G. asclepiadea was neither cytotoxic nor genotoxic in a wide range of concentrations (0.25-2.5 mg/ml) and thus can be used to further investigate potential beneficial usage in pharmacology and medicine. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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