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Vibriosis caused by the pathogenic bacterium Vibrio (Listonella) anguillarum leads to serious losses in European sea bass, Dicentrarchus labrax. Because of its pleiotropic activity in controlling immune and inflammatory responses against various pathogens, interleukin-1beta (IL-1beta) is an attractive candidate for resistance to bacterial vibriosis. Four polymorphisms c.76 + 52CT, c.76 + 157AG, c.76 + 215Aand c76 + 310AG of IL1B were genotyped in progeny of four families of wild sea bass captured in geographically distinct regions of the Black Sea and Sea of Azov and challenged with V. anguillarum. In the transmission disequilibrium test, the TGGG haplotype of IL1B showed significant overtransmission from parents to surviving progeny, thereby suggesting an association with higher resistance to V. anguillarum infection (Odds Ratio 0.38, P10(-7)). Using a luciferase reporter assay, we found a 1.4-fold increase in transcription activity of the protective IL1B TGGG variant compared to the susceptible CAAA variant of IL1B. The higher transcriptional activity of IL1B TGGG may arise from the functional effects of c.76 + 157AG and c.76 + 215AG polymorphisms disrupting potential binding sites for glucocorticoid receptor and YY1, both are negative transcription regulators. |
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