Bone morphogenetic protein-2: a potential regulator in scleral remodeling
| Autor: | Jianmin, Hu, Dongmei, Cui, Xiao, Yang, Shaowei, Wang, Shoulong, Hu, Chuanxu, Li, Junwen, Zeng |
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| Rok vydání: | 2008 |
| Předmět: |
Adult
Cytoplasm Tissue Inhibitor of Metalloproteinase-2 Time Factors Adolescent Cell Cycle Bone Morphogenetic Protein 2 Fluorescent Antibody Technique Fibroblasts Gene Expression Regulation Enzymologic Recombinant Proteins Culture Media Organ Specificity Transforming Growth Factor beta Bone Morphogenetic Proteins Humans Matrix Metalloproteinase 2 RNA Messenger Cells Cultured Sclera Cell Proliferation Research Article |
| Zdroj: | Molecular Vision |
| ISSN: | 1090-0535 |
| Popis: | Purpose Bone morphogenetic protein 2 (BMP-2) is a member of the main subgroup of bone morphogenetic proteins within the transforming growth factor-β superfamily. BMP-2 is involved in numerous cellular functions including development, cell proliferation, apoptosis, and extracellular matrix synthesis. We examined BMP-2 expression in human scleral fibroblasts (HSF) and assessed the effects of recombinant human BMP-2 (rhBMP-2) on HSF proliferation, matrix metalloproteinase-2 (MMP-2), and tissue inhibitor of metalloproteinase-2 (TIMP-2). Methods We used confocal fluorescence microscopy (CFM) to study BMP-2 distribution in HSF cells and frozen human scleral sections. The influence of rhBMP-2 on cell proliferation at different concentrations (0 ng/ml, 1 ng/ml, 10 ng/ml, and 100 ng/ml) was evaluated by the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. The effects of rhBMP-2 on the cell cycle were investigated with flow cytometric analysis. Reverse transcription polymerase chain reaction (RT–PCR) and enzyme-linked immunosorbent assay (ELISA) were used to examine MMP-2 and TIMP-2 mRNAs and secreted proteins in HSF that were incubated with rhBMP-2. Results BMP-2 protein expression from human sclera was confirmed by CFM. Cell proliferation was significantly increased with 100 ng/ml rhBMP-2 in a time-dependent manner (p |
| Databáze: | OpenAIRE |
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