Multiple Aspects of PIP2 Involvement in
| Autor: | Livia, Ulicna, Jana, Rohozkova, Pavel, Hozak |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Cell Nucleus
Phosphatidylinositol 4 5-Diphosphate Proteasome Endopeptidase Complex nucleus Gene Expression Regulation Developmental Proteins Leucine-Rich Repeat Proteins Chromosomes Gametogenesis Article Phosphotransferases (Alcohol Group Acceptor) phosphatidylinositol 4 5-bisphosphate C. elegans Animals lipids (amino acids peptides and proteins) Hermaphroditic Organisms RNA Interference Meiotic Prophase I Caenorhabditis elegans Caenorhabditis elegans Proteins PPK-1 |
| Zdroj: | International Journal of Molecular Sciences |
| ISSN: | 1422-0067 |
| Popis: | One of the most studied phosphoinositides is phosphatidylinositol 4,5-bisphosphate (PIP2), which localizes to the plasma membrane, nuclear speckles, small foci in the nucleoplasm, and to the nucleolus in mammalian cells. Here, we show that PIP2 also localizes to the nucleus in prophase I, during the gametogenesis of C. elegans hermaphrodite. The depletion of PIP2 by type I PIP kinase (PPK-1) kinase RNA interference results in an altered chromosome structure and leads to various defects during meiotic progression. We observed a decreased brood size and aneuploidy in progeny, defects in synapsis, and crossover formation. The altered chromosome structure is reflected in the increased transcription activity of a tightly regulated process in prophase I. To elucidate the involvement of PIP2 in the processes during the C. elegans development, we identified the PIP2-binding partners, leucine-rich repeat (LRR-1) protein and proteasome subunit beta 4 (PBS-4), pointing to its involvement in the ubiquitin–proteasome pathway. |
| Databáze: | OpenAIRE |
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