| Autor: |
Alexander, Heldner, Matthew D, Heath, Benjamin, Schnautz, Sebastian, Kotz, Adam, Chaker, Matthias F, Kramer, Constanze A, Jakwerth, Ulrich M, Zissler, Carsten B, Schmidt-Weber, Simon, Blank |
| Rok vydání: |
2022 |
| Zdroj: |
Probiotics and antimicrobial proteins. |
| ISSN: |
1867-1314 |
| Popis: |
Sensing of the intestinal microbiota by the host immune system is important to induce protective immune responses. Hence, modification of the gut microbiota might be able to prevent or treat allergies, mediated by proinflammatory Th2 immune responses. The aim was to investigate the ex vivo immunomodulatory effects of the synbiotics Pollagen® and Kallergen®, containing the probiotic bacterial strains Lactobacillus, Lacticaseibacillus and Bifidobacterium, in the context of grass pollen allergy. Peripheral blood mononuclear cells (PBMCs) from grass pollen-allergic patients and healthy controls were stimulated with grass pollen extract (GPE) and synbiotics and Gata3 expression and cytokine secretion analyzed. Monocyte-derived dendritic cells (MoDCs) cells were matured in the presence of GPE and synbiotics, co-cultured with autologous naïve T cells and maturation markers and cytokine secretion analyzed. GPE stimulation of PBMCs from grass pollen-allergic patients resulted in a significant higher production of the Th2 cytokines IL-4, IL-5, IL-9 and IL-13 compared to healthy controls. Gata3 |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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