A translocator protein ligand PK11195 shows antigrowth activity in human choriocarcinoma cells
| Autor: | Noriyuki, Takai, Naoko, Kira, Terukazu, Ishii, Toshie, Yoshida, Masakazu, Nishida, Yoshihiro, Nishida, Kaei, Nasu, Masayuki, Takano, Haruna, Midori, Satoko, Koga, Hisashi, Narahara |
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| Rok vydání: | 2012 |
| Předmět: |
Caspase 7
Membrane Potential Mitochondrial Caspase 3 Cell Survival Hydatidiform Mole Invasive Cell Cycle Antineoplastic Agents Apoptosis Cell Cycle Proteins Isoquinolines Ligands Enzyme Activation Gene Expression Regulation Neoplastic Receptors GABA Pregnancy Cell Line Tumor Uterine Neoplasms Humans Female Apoptosis Regulatory Proteins Cell Proliferation |
| Zdroj: | Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 33(5) |
| ISSN: | 1423-0380 |
| Popis: | The potential anticancer agent 1-(2-chlorophenyl-N-methylpropyl)-3-isoquinolinecarboxamide (PK11195), a translocator protein ligand (initially described as a ligand for the peripheral benzodiazepine receptor), induces apoptosis in some lines of human tumor cells. We investigated the effect of PK11195 in the choriocarcinoma cell line, BeWo. BeWo cells were treated with various concentrations of PK11195, and changes in cell growth, the cell cycle, apoptosis, and related parameters were examined. A WST-1 assay showed that BeWo cells were sensitive to the growth inhibitory effect of PK11195. In contrast, the nonsite selective ligand diazepam has a little effect on these cells. Cell cycle analysis indicated that exposure to PK11195 decreased the proportion of cells in the S phase and increased the proportion in the G0/G1 phases of the cell cycle. Induction of apoptosis was confirmed by Annexin V staining of externalized phosphatidylserine, by the loss of mitochondrial transmembrane potential, and by antibodies directed against histones from fragmented DNA. This induction occurred in conjunction with the altered expression of genes related to cell growth, malignant phenotype, and apoptosis. These results suggest that PK11195 may serve as a therapeutic agent for the treatment of choriocarcinoma. |
| Databáze: | OpenAIRE |
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