Structure determination of two conotoxins from Conus textile by a combination of matrix-assisted laser desorption/ionization time-of-flight and electrospray ionization mass spectrometry and biochemical methods

Autor:	D E, Kalume, J, Stenflo, E, Czerwiec, B, Hambe, B C, Furie, B, Furie, P, Roepstorff
Rok vydání:	2000
Předmět:	Alkylation Hydrolysis Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Molecular Sequence Data Mollusk Venoms Amino Acid Sequence Disulfides Amino Acids Conotoxins Oxidation-Reduction Mass Spectrometry
Zdroj:	Journal of mass spectrometry : JMS. 35(2)
ISSN:	1076-5174
Popis:	Two highly modified conotoxins from the mollusc Conus textile, epsilon-TxIX and Gla(1)-TxVI, were characterized by matrix-assisted laser desorption/ionization and electrospray mass spectrometry and also by electrospray ionization tandem and triple mass spectrometry in combination with enzymatic cleavage and chemical modification reactions. The mass spectrometric studies allowed the confirmation of the sequence determined by Edman degradation and assignment of unidentified amino acid residues, among which bromotryptophan residues and an O-glycosylated threonine residue were observed. Methyl esterification was found necessary for the site-specific assignment of the Gla residues in the peptides.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::ea0e6a98db9b624726333401131a7430 https://pubmed.ncbi.nlm.nih.gov/10679974 Zobrazit plný text záznamu Plný text