| Autor: |
Pompeyo R, Quesada, Erick, Riquelme, Jared, Burks, Amanda, Rakoski, Ismet, Sahin, Florencia, McAllister |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 2435 |
| ISSN: |
1940-6029 |
| Popis: |
We describe the staining methods used for simultaneous detection of tumor microenvironment components as well as the automated quantification methodologies. This method uses mouse formalin-fixed paraffin-embedded tissues and multiplex immunofluorescence (Multiplex IF) followed by multispectral imaging. Currently, this methodology has shown to have a valuable role in murine immunoprofiling, and can be useful when evaluating the changes incurred on the tumor microenvironment upon various immunopreventive strategies. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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