[Nucleic acids interactions with short oligonucleotide derivatives. II. Tandem of short oligonucleotides as highly sensitive system for identification of single base substitutions in target DNA]

Autor:	D V, Pyshnyĭ, M A, Podyminogin, I A, Pyshnaia, S G, Lokhov, E M, Ivanova, V F, Zarytova
Rok vydání:	1997
Předmět:	Alkylating Agents Oligonucleotides Temperature Autoradiography Point Mutation Electrophoresis Polyacrylamide Gel DNA Nucleic Acid Denaturation Phosphorus Radioisotopes
Zdroj:	Bioorganicheskaia khimiia. 23(7)
ISSN:	0132-3423
Popis:	A new approach for modification of target DNAs with tandems of derivatives of short oligonucleotides was suggested that allows highly selective modification of perfect duplexes only. At physiological temperatures, the efficiency of DNA modification by a dodecanucleotide alkylating agent was demonstrated to be the same for both perfect and mismatch-containing duplexes, whereas the tetranucleotide reagent in the presence of two flanking effectors alkylated with high selectivity the target DNA in the perfect duplex only.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::e9ecdd0164dd1007b285642a8972df7c https://pubmed.ncbi.nlm.nih.gov/9471976 Zobrazit plný text záznamu