Silencing the gene encoding C/EBP homologous protein lessens acute brain injury following ischemia/reperfusion☆

Autor:	Wang, Fengzhang, Zhang, Yuan, He, Chunke, Wang, Tingting, Piao, Qiyan, Liu, Qun
Jazyk:	angličtina
Rok vydání:	2012
Předmět:	Research and Report Article: Brain Injury and Neural Regeneration subarachnoid hemorrhage interleukin-1β endoplasmic reticulum stress tumor necrosis factor-α cerebral infarction neural regeneration Alzheimer’s disease ischemia/reperfusion C/EBP homologous protein
Zdroj:	Neural Regeneration Research
ISSN:	1876-7958 1673-5374
Popis:	C/EBP homologous protein, an important transcription factor during endoplasmic reticulum stress, participates in cell apoptosis mediated by endoplasmic reticulum stress. Previous studies have shown that C/EBP homologous protein mediates nerve injury during Alzheimer's disease, subarachnoid hemorrhage and spinal cord trauma. In this study, we introduced C/EBP homologous protein short hairpin RNA into the brains of ischemia/reperfusion rat models via injection of lentiviral vector through the left lateral ventricle. Silencing C/EBP homologous protein gene expression significantly reduced cerebral infarction volume, decreased water content and tumor necrosis factor-α and interleukin-1β mRNA expression in brain tissues following infarction, diminished the number of TUNEL-positive cells in the infarct region, decreased caspase-3 protein content and increased Bcl-2 protein content. These results suggest that silencing C/EBP homologous protein lessens cell apoptosis and inflammatory reactions, thereby protecting nerves.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::e9e55a6ebf85a73db646e9cedbeda473 http://europepmc.org/articles/PMC4200717 Zobrazit plný text záznamu