| Autor: |
V I, Turilova, T D, Smirnova, M P, Samoĭlovich, T R, Sukhikh |
| Rok vydání: |
1999 |
| Předmět: |
|
| Zdroj: |
Tsitologiia. 41(10) |
| ISSN: |
0041-3771 |
| Popis: |
The IgE content in both cytoplasm and culture medium of U 266 myeloma cells, was studied by the enzyme-linked immunoassay in correlation with Ag-staining of NORs of chromosomes in their nuclei throughout 9 days after cell seeding. Proliferative activity of the cells was evaluated with 3H-thymidine labeling. The average values of both the cytoplasmic IgE content and its secretion level in U 266 cell population, being in logarithmic growth phase, were higher in S-phase cells as compared with G1-cells. On comparison of results of the present and previous (Turilova et al., 1998) studies it was revealed that U 266 myeloma cell line had a high stability of cell proliferation kinetics and IgE secretion and the dynamics of Ag-NORs-staining, while the number of argentophilic grains in the cell nuclei in the present experiment was higher to correlate with an enhanced IgE production. It is suggested that Ag-NORs-staining reflects the level of specific functional activity of cells in the U 266 line. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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