Sialidase activity in normal and atherosclerotic human aortic intima

Autor:	N K, Golovanova, E V, Gracheva, O P, Il'inskaya, E M, Tararak, N V, Prokazova
Rok vydání:	2002
Předmět:	Adult Male Azides Arteriosclerosis Cell Membrane Neuraminidase Enzyme-Linked Immunosorbent Assay G(M1) Ganglioside Middle Aged Ammonium Chloride Gangliosides Sialic Acids Humans Female Tunica Intima Aorta Aged
Zdroj:	Biochemistry. Biokhimiia. 67(11)
ISSN:	0006-2979
Popis:	Sialidase activity has been determined in homogenates of human aortic intima by measuring the amount of GM1 formed during the incubation of ganglioside GD1a with the tissue homogenates. Areas with atherosclerotic lesions as well as adjacent areas without histological evidence of atherosclerosis were taken for comparison. The rate of GM1 formation from GD1a in the presence of homogenates of the atherosclerotic intima was 20 pmol/h per mg protein. Homogenates of the unaffected intima did not desialylate GD1a. Sialidase activity of the atherosclerotic intima was linear for 1.5 h at GD1a content up to 1.5 nmol and at homogenate protein up to 1 micro g. NH4Cl and NeuAc2en, inhibitors of lysosomal function and plasma membrane-bound sialidase, respectively, reduced sialidase activity of homogenates of the atherosclerotic intima by 94%. The results indicate that atherosclerotic lesions and unaffected intima differ in their activity and specificity of sialidases that cleave gangliosides.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::e9c73864eb49d8478d3cf0cb2eb41d58 https://pubmed.ncbi.nlm.nih.gov/12495418 Zobrazit plný text záznamu Full text from SpringerLink