| Autor: |
A P, Gee, C, Lee, J W, Sleasman, M, Madden, J, Ugelstad, D J, Barrett |
| Rok vydání: |
1987 |
| Předmět: |
|
| Zdroj: |
Bone marrow transplantation. 2(2) |
| ISSN: |
0268-3369 |
| Popis: |
It has previously been demonstrated that graft-versus-host disease can be overcome in patients receiving HLA-mismatched bone marrow transplants by prior in vitro depletion of T lymphocytes from the marrow. In this report we describe the use of monoclonal antibodies and magnetic microspheres for the depletion of T cells from peripheral blood and bone marrow. The target cells are sensitized with antibodies directed against the CD2, CD3, CD4 and/or CD8 cell surface antigens, captured by magnetic beads coated with sheep anti-mouse IgG antibody and collected by placing the cell suspension in a magnetic field. This simple, rapid procedure results in the efficient removal of T cells from peripheral blood and from bone marrow without affecting the colony-forming potential of normal hematopoietic stem cells. The procedure is capable of being scaled up for the treatment of larger volumes of marrow that are required for clinical transplantation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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