| Autor: |
Fei, Ruan, Rui-jin, Wu, Kai-hong, Xu, Jun-yan, Ma, Dong-hong, Lu, Li-qing, Chen, Fei-xia, Tu, Zheng, Liang, Jun, Lin |
| Rok vydání: |
2011 |
| Předmět: |
|
| Zdroj: |
Zhonghua yi xue za zhi. 91(23) |
| ISSN: |
0376-2491 |
| Popis: |
To evaluate the effects of PRL-3 siRNA (small interfering RNA) on the migration of endometriotic stromal cells.Primary endometriotic stromal cells were cultured in vitro. Then PRL-3 (phosphatase of regenerating liver-3) siRNA was transfected to silence the PRL-3 gene. And the PRL-3 protein expression was analyzed by Western blot. The changes of cell migration were detected by cell pseudopod formation, scratch test and transwell cell migration test.The expression of PRL-3 protein significantly decreased in the experimental group versus two other control groups (P0.05). The formation of cell pseudopod was much less in experimental group than that in control groups. Within the same time, the number of migration cells was 0.87 ± 0.21 in experimental group. It was less than 1.75 ± 0.28 in blank control group and 1.63 ± 0.39 in negative control group (P0.05).PRL-3 siRNA can down-regulate the PRL-3 gene and decrease the migratory capacity of endometriotic stromal cells. And PRL-3 may be a promising target in the therapeutics of endometriosis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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