CRISPRTarget
| Autor: | Biswas, Ambarish, Gagnon, Joshua N., Brouns, Stan J.J., Fineran, Peter C., Brown, Chris M. |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Archaeal Viruses
Streptococcus Phages Archaeal Proteins CRISPR-Associated Proteins Molecular Sequence Data Evolution Molecular Bacterial Proteins Streptococcus thermophilus Bacteriophages Clustered Regularly Interspaced Short Palindromic Repeats crRNA Base Pairing Bacteria Base Sequence small RNA targets Cas Computational Biology bioinformatics RNA Bacterial phage resistance CRISPR Sulfolobus solfataricus horizontal gene transfer R-loop DNA Intergenic Sequence Alignment Research Paper |
| Zdroj: | RNA Biology |
| ISSN: | 1555-8584 1547-6286 |
| Popis: | The bacterial and archaeal CRISPR/Cas adaptive immune system targets specific protospacer nucleotide sequences in invading organisms. This requires base pairing between processed CRISPR RNA and the target protospacer. For type I and II CRISPR/Cas systems, protospacer adjacent motifs (PAM) are essential for target recognition, and for type III, mismatches in the flanking sequences are important in the antiviral response. In this study, we examine the properties of each class of CRISPR. We use this information to provide a tool (CRISPRTarget) that predicts the most likely targets of CRISPR RNAs (http://bioanalysis.otago.ac.nz/CRISPRTarget). This can be used to discover targets in newly sequenced genomic or metagenomic data. To test its utility, we discover features and targets of well-characterized Streptococcus thermophilus and Sulfolobus solfataricus type II and III CRISPR/Cas systems. Finally, in Pectobacterium species, we identify new CRISPR targets and propose a model of temperate phage exposure and subsequent inhibition by the type I CRISPR/Cas systems. |
| Databáze: | OpenAIRE |
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