| Autor: |
V D, Pivovarov, D Yu, Ryazantsev, M A, Simonova, T V, Yegorova, S V, Khlgatian, S K, Zavriev, E V, Svirshchevskaya |
| Rok vydání: |
2017 |
| Předmět: |
|
| Zdroj: |
Molekuliarnaia biologiia. 52(4) |
| ISSN: |
0026-8984 |
| Popis: |
Successful disease prevention and therapy critically depend on timely diagnosis of infections. Quantitative immuno-PCR (qiPCR) technology improves the sensitivity in the detection of antibodies to pathogens. A qiPCR-based assay was developed to determine IgG antibodies to Epstein-Barr virus (EBV) in the human blood serum. EBV nuclear protein 1 fragment (pEBV) was expressed in Escherichia coli. A synthetic single-stranded deoxyribonucleotide was conjugated to streptavidin, and the conjugate was used to detect рEBV-IgG1-biotin complexes by qiPCR. The IgG1 titers determined by qiPCR were compared to the results of enzyme-linked immunosorbent assay (ELISA). The sensitivity of qiPCR was one order of magnitude higher than that of ELISA. Thus, a highly sensitive qiPCR-based assay was developed to quantitate antibodies specific to the recombinant EBV antigen. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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