| Autor: |
Maulik S, Jani, Aneesh T, Veetil, Yamuna, Krishnan |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Methods in enzymology. 638 |
| ISSN: |
1557-7988 |
| Popis: |
Membrane-initiated steroid signaling (MISS) involves rapid second messenger based intracellular signaling without coupling to transcription or translation. MISS activates important cellular signaling cascades such as mitogen-activated protein kinase (MAPK) or adenylate cyclase pathways. Despite its vital role in signaling, the downstream second messengers involved in MISS and their temporal dynamics remain elusive. A technology which can offer pristine spatiotemporal control over the release of the steroid initiator could pave the way to understand these rapid and ultrasensitive signaling processes. Toward this, we describe a DNA-nanocapsule based technology to chemically release steroids and study MISS in endothelial cells. Here we discuss the synthesis and cellular protocols for investigators who seek to utilize DNA-nanocapsules for the chemically triggered release of small molecules. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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