| Autor: |
Ajaz, Ahmad, Rao Muzaffar A, Khan, Khalid M, Alkharfy, Mohammad, Raish, Fahad I, Al-Jenoobi, Abdullah M, Al-Mohizea |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
Natural product communications. 10(8) |
| ISSN: |
1934-578X |
| Popis: |
Glibenclamide and thymoquinone plasma concentrations were analysed using a sensitive RP-HPLC method, and non-compartmental model pharmacokinetic parameters were calculated. The maximum reduction in blood glucose level was observed 3 hours following glibenclamide administration, which reached 47.4% of baseline, whereas it was reduced by 53.0% to 56.2% when co-administrated with thymoquinone. Plasma concentration of glibenclamide was increased by 13.4% and 21.8% by the co-administration of thymoquinone as single and multiple doses, respectively (P0.05). The AUC and TI/2 of glibenclamide were also increased respectively by 32.0% and 17.4% with a thymoquinone single dose, and by 52.5% and 92.8% after chronic treatment. Furthermore, diabetic rats treated with thymoquinone demonstrated a marked decrease in hepatic protein expressions of CYP3A2 and CYP2C 11 enzymes that are responsible for the metabolism of glibenclamide. The current data suggest that thymoquinone exhibits a synergistic effect with glibenclamide on glucose level, which could be explained by reducing CYP450 activity at the protein level. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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