| Autor: |
Nuo, Zhou, Xuan-ping, Huang, Ni, Liao, Shan-liang, Wei, Fei-xin, Liang, Hua-ming, Mai |
| Rok vydání: |
2007 |
| Předmět: |
|
| Zdroj: |
Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology. 25(5) |
| ISSN: |
1000-1182 |
| Popis: |
To clone human bone morphogenetic protein-2 (hBMP2) gene and construct its eukaryotic expression vector pcDNA3.1 -hBMP2.Human BMP2 gene was amplified by RT-PCR method from human osteosarcoma cells and constructed into eukaryotic expression vector pcDNA3.1-hBMP2. The gene in the vector pcDNA3.1-hBMP2 was identified by PCR amplification, enzyme digestion and DNA sequencing.The cloned DNA was confirmed to be hBMP-2 gene.In this study, hBMP2 gene is successfully cloned and its eukaryotic expression vector pcDNA3.1-hBMP2 is constructed, which provides the foundation of using BMP2 gene therapy to accelerate new bone formation in distraction osteogenesis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
