Chemical synthesis of lymphotactin: a glycosylated chemokine with a C-terminal mucin-like domain

Autor:	L A, Marcaurelle, L S, Mizoue, J, Wilken, L, Oldham, S B, Kent, T M, Handel, C R, Bertozzi
Rok vydání:	2001
Předmět:	Lymphokines Glycosylation Magnetic Resonance Spectroscopy Protein Conformation Sialoglycoproteins Molecular Sequence Data Membrane Proteins Receptors Cell Surface Kidney Biochemistry Chemokines C Receptors G-Protein-Coupled Humans Amino Acid Sequence Cells Cultured
Zdroj:	Chemistry (Weinheim an der Bergstrasse, Germany). 7(5)
ISSN:	0947-6539
Popis:	The synthesis of a 93-residue chemokine, lymphotactin, containing eight sites of O-linked glycosylation, was achieved using the technique of native chemical ligation. A single GalNAc residue was incorporated at each glycosylation site using standard Fmoc-chemistry to achieve the first total synthesis of a mucin-type glycoprotein. Using this approach quantities of homogeneous material were obtained for structural and functional analysis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::e81611a74fcc883fdda9d2a9db3529ef https://pubmed.ncbi.nlm.nih.gov/11303872 Zobrazit plný text záznamu Plný text