| Autor: |
Min, Jia, Tie-hong, Yang, Xiu-juan, Yao, Jia, Meng, Jing-ru, Meng, Qi-bing, Mei |
| Rok vydání: |
2007 |
| Předmět: |
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| Zdroj: |
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials. 30(2) |
| ISSN: |
1001-4454 |
| Popis: |
To investigate the anti-oxidative effect of Angelica polysaccharide sulphate( APS).The Hela cells were cultured conventionally. Then APS was added and cultured together in different concentration for 24h followed by a oxidative injury with H2O2 or UV irradiation. The anti-oxidative effects of APS were detected as follow: cell viability was measured by MTT assay; colorimetric analysis was used to determine SOD activity, GSH and MDA level in cytoplasm.Treatment of H2O2 or UV irradiation significantly decreased cell viability, GSH and SOD activity in cytoplasm,while increased MDA in cytoplasm. At the range of 0. 3 -100microg/ml, APS significantly increased cell viabilty, GSH and SOD activity,while decreased MDA in a dose dependent manner( P0. 05 or P0.01).APS has anti-oxidative effect,which may be one of its anti-AIDS mechanisms. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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