| Autor: |
Nuoendagula, Narushima, Mana, Uesugi, Mikiko, Murai, Yasushi, Katayama, Yoshihiro, Iimura, Yosuke, Kajita, Shinya |
| Jazyk: |
angličtina |
| Rok vydání: |
2017 |
| Předmět: |
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| Popis: |
In this study, we devised a method for the in vitro regeneration and subsequent genetic transformation of male sterile marigold. To our knowledge, this is the first report of generation of transgenic plants with a single genotype of marigold via Agrobacterium-mediated transformation. We obtained four transgenic lines from two independent experiments with 496 leaf explants, which were inoculated by an Agrobacterium strain LBA4404 harboring the plasmid, pIG121-Hm. Although the efficiency of the transformation in our system was low, stable expression of uidA gene in adventitious shoots and compound leaves could be detected in β-glucuronidase histochemical analysis. This protocol contributes to the progress of genetic studies and molecular breeding of this species. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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