| Autor: |
J K, Franz, S A, Kolb, K M, Hummel, F, Lahrtz, M, Neidhart, W K, Aicher, T, Pap, R E, Gay, A, Fontana, S, Gay |
| Rok vydání: |
1998 |
| Předmět: |
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| Zdroj: |
European journal of immunology. 28(9) |
| ISSN: |
0014-2980 |
| Popis: |
The massive infiltration of synovium with CD4+ T cells during the course of rheumatoid arthritis (RA) implies the expression of chemoattractant factors by resident synovial cells. Therefore, we analyzed the expression of IL-16, a potent chemoattractant for CD4+ T cells, to account for the accumulation of CD4+ T cells in RA. Indeed, IL-16 was found to be significantly elevated in synovial fluid (SF) from patients with RA as compared to non-RA arthritis (p0.001), osteoarthritis (p0.001) and controls (p0.001). Chemotaxis studies showed IL-16 to contribute to the strong chemotactic activities of RA-SF. In situ hybridization (ISH) revealed IL-16 mRNA-expressing cells located within the lining layer of rheumatoid synovial tissue. In the sublining area, only scattered IL-16 transcript-positive cells could be detected, mainly adjacent to blood vessels. By a double-labeling technique, combining ISH for IL-16 mRNA and immunohistochemistry for CD68, synovial fibroblast-like, CD68-negative cells were identified as a major source of IL-16 mRNA within RA synovium. This study demonstrates that synovial fibroblasts produce IL-16 in RA and thus mediate chemoattraction of CD4+ cells into synovial tissue. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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