| Autor: |
Atsushi, Wada, Mitsutomo, Kohno, Kana, Oiwa, Ryo, Hashimoto, Takahisa, Koizumi, Yusuke, Nakamura, Tomoki, Nakagawa, Ryota, Masuda, Kichizo, Kaga, Masayuki, Iwazaki |
| Rok vydání: |
2017 |
| Předmět: |
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| Zdroj: |
The Tokai journal of experimental and clinical medicine. 42(4) |
| ISSN: |
2185-2243 |
| Popis: |
Ischemia-reperfusion injury resulting in post-transplant lung dysfunction involves a complicated network of cytokines that has yet to be fully investigated. We analyzed temporal changes in cytokine levels in epithelial lining fluid (ELF) from the distal airways of a canine lung transplantation model.Ischemic time was set to 18 hours to enhance lung injury in a left single-lung transplantation model. ELF was collected via bronchoscopic microsampling, a minimally-invasive technique allowing repeated sampling, hourly up to 5 hours after reperfusion started. We compared levels of pro-inflammatory cytokines in ELF with those at baseline (time zero), and with a sham-operated control group.Concentrations of tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) significantly increased immediately after the start of lung reperfusion in the transplant group relative to the sham group (P0.005 and P0.05, respectively); both were sustained through the 5 hours. Interferon gamma (IFN-γ levels were significantly reduced 3 h after reperfusion started (P0.05).We found time- and cytokine-dependent changes in TNF-α, IL-6, and IFN-γ in ELF from the lung transplantation model. These specific changes in the cytokine profile may relate to the mechanism underlying post-transplant lung dysfunction. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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