[Relation between DNA replication and neutral Mn 2+-dependent DNAse activity in chromatin]

Autor: V G, Vinter, A N, Askarova, N L, Zotkina, V V, Kulikov, O B, Drize, M A, Shliankevich
Rok vydání: 1990
Předmět:
Zdroj: Biokhimiia (Moscow, Russia). 55(1)
ISSN: 0320-9725
Popis: In cultures of primary murine fibroblasts the 10% serum stimulates the replicative synthesis of DNA inhibited by aphidicolin and araC (cytosine arabinoside). Using direct immunofluorescence analysis, it was shown that antibodies penetrate inside the cells and after 4 hours are pooled in the nuclei, where they remain for another 20 hours. The substitution of antibodies against chromatin DNAase by bovine serum albumin of normal serum gamma-globulins does not interfere with the DNA synthesis induction.
Databáze: OpenAIRE