| Autor: |
Carolina, Oliveira-Rizzo, María Carolina, Ottati, Rafael Sebastián, Fort, Santiago, Chavez, Juan Manuel, Trinidad, Andrés, DiPaolo, Beatriz, Garat, José Roberto, Sotelo-Silveira, María Ana, Duhagon |
| Rok vydání: |
2021 |
| Zdroj: |
Non-coding RNA. 8(1) |
| ISSN: |
2311-553X |
| Popis: |
Prostate cancer is a major health problem worldwide. MiR-183 is an oncomiR and a candidate biomarker in prostate cancer, affecting various pathways responsible for disease initiation and progression. We sought to discover the most relevant processes controlled by miR-183 through an unbiased transcriptomic approach using prostate cell lines and patient tissues to identify miR-183 responsive genes and pathways. Gain of function experiments, reporter gene assays, and transcript and protein measurements were conducted to validate predicted functional effects and protein mediators. A total of 135 candidate miR-183 target genes overrepresenting cell adhesion terms were inferred from the integrated transcriptomic analysis. Cell attachment, spreading assays and focal adhesion quantification of miR-183-overexpressing cells confirmed the predicted reduction in cell adhesion. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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