A fast, inexpensive, and safe method for residue analysis of meptyldinocap in different fruits by liquid chromatography/tandem mass spectrometry
| Autor: | Kaushik, Banerjee, Soma, Dasgupta, Manjusha R, Jadhav, Dattatraya G, Naik, Axel Patrick, Ligon, Dasharath P, Oulkar, Rahul H, Savant, Pandurang G, Adsule |
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| Rok vydání: | 2011 |
| Předmět: |
Magnetic Resonance Spectroscopy
Formates Spectrophotometry Infrared Hydrolysis Pesticide Residues Reproducibility of Results Acetates Reference Standards Fungicides Industrial Solutions Dinitrobenzenes Kinetics Tandem Mass Spectrometry Fruit Sample Size Costs and Cost Analysis Solvents Thermodynamics Indicators and Reagents Spectrophotometry Ultraviolet Chromatography High Pressure Liquid |
| Zdroj: | Journal of AOAC International. 93(6) |
| ISSN: | 1060-3271 |
| Popis: | An analytical method is reported for residue analysis of the fungicide meptyldinocap in different fruit matrixes that involves extraction with ethyl acetate, hydrolysis of the residues with ethanolamine, and determination by LC/MS/MS. The method involves extraction of 10 g sample with 10 mL ethyl acetate; evaporation of the ethyl acetate phase to dryness, and subsequent hydrolysis of the residues to 4,6-dinitro-2-(1-methylheptyl) phenol on reaction with 1% ethanolamine. The pH of this hydrolyzed product was neutralized with formic acid and analyzed by LC/MS/MS. The hydrolysis reaction followed pseudo-first-order kinetics, and the reaction product was spectroscopically confirmed as 2-(1-methylheptyl)-4,6-dinitrophenol. The method offered80% recoveries at an LOQ of 10 ng/g for grape and mango, 25 ng/g for pomegranate with intralaboratory Horwitz ratio0.5, and measurement uncertainties10% at LOQ levels. Considering first-order rate kinetics, activation energy, enthalpy of activation, and entropy of activation varied as solventmangograpepomegranate. Free energy of activation at 298 K was higher than at 280 K and was similar for solvent and three matrixes at both temperatures. |
| Databáze: | OpenAIRE |
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