Validation of a microscale extraction and high-throughput UHPLC-QTOF-MS analysis method for huperzine A in Huperzia

Autor:	Daniel, Cuthbertson, Jasenka, Piljac-Žegarac, Bernd Markus, Lange
Rok vydání:	2011
Předmět:	Alkaloids Limit of Detection Plant Extracts Linear Models Reproducibility of Results Huperzia Sesquiterpenes Chromatography High Pressure Liquid Article High-Throughput Screening Assays
Zdroj:	Biomedical chromatography : BMC. 26(10)
ISSN:	1099-0801
Popis:	Herein we report on an improved method for the microscale extraction of huperzine A (HupA), an acetylcholinesterase-inhibiting alkaloid, from as little as 3 mg of tissue homogenate from the clubmoss Huperzia squarrosa (G. Forst.) Trevis with 99.95 % recovery. We also validated a novel UHPLC-QTOF-MS method for the high-throughput analysis of H. squarrosa extracts in only 6 min, which, in combination with the very low limit of detection (20 pg on column) and the wide linear range for quantification (20 to 10,000 pg on column), allow for a highly efficient screening of extracts containing varying amounts of HupA. Utilization of this methodology has the potential to conserve valuable plant resources.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::e61be5e1435129fec743c64d9e3288a9 https://pubmed.ncbi.nlm.nih.gov/22275140 Zobrazit plný text záznamu Plný text