Stages of T cell activation: continued antigen dependence of IL 2-producing cells after IL 2 receptor expression
| Autor: | R A, Miller, M K, Rozans, A A, Ythier, T B, Strom |
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| Rok vydání: | 1986 |
| Předmět: |
Isoantigens
Mice Inbred BALB C Time Factors H-2 Antigens Receptors Interleukin-2 Cell Separation T-Lymphocytes Helper-Inducer Cross Reactions Lymphocyte Activation Mice Inbred C57BL Epitopes Mice Mice Inbred CBA Animals Humans Interleukin-2 Lymphocyte Culture Test Mixed Receptors Immunologic Histocompatibility Antigen H-2D |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 136(3) |
| ISSN: | 0022-1767 |
| Popis: | Alloantigen stimulation leads, within 48 to 72 hr, to the expression of IL 2 receptors (IL 2R) on the surface of most of the helper T cells with specificity for the stimulating antigens. The IL 2R-bearing cells, separated by flow cytometry from 3-day human or mouse mixed lymphocyte cultures, were found by limiting dilution methods to be enriched 10- to 20-fold (compared to IL 2R- cells) for antigen-specific helper T cells detected by IL 2 production. Although these cells have been activated to an IL 2R+ stage, most of them are unable to produce detectable IL 2 unless they are cultured together with the original, activating antigen. Even when IL 2 is supplied, and lymphokine production measured by assay of a second factor, IL 3, the large majority of individual activated helper T cells remain dependent on further antigenic exposure for their continued maturation into lymphokine-secreting effectors. Helper T cells at this early stage of activation can therefore proliferate when given IL 2 alone, but lymphokine secretion involves a second antigen-dependent step. |
| Databáze: | OpenAIRE |
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