Protein kinase Cdelta activation induces apoptosis in response to cardiac ischemia and reperfusion damage: a mechanism involving BAD and the mitochondria
| Autor: | Christopher L, Murriel, Eric, Churchill, Koichi, Inagaki, Luke I, Szweda, Daria, Mochly-Rosen |
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| Rok vydání: | 2004 |
| Předmět: |
Male
Myocardial Ischemia Poly (ADP-Ribose) Polymerase-1 bcl-X Protein Apoptosis In Vitro Techniques Protein Serine-Threonine Kinases Proto-Oncogene Proteins In Situ Nick-End Labeling Animals Humans Rats Wistar Protein Kinase Inhibitors Protein Kinase C Caspase 3 Myocardium Cytochromes c Proteins Mitochondria Rats Enzyme Activation Protein Kinase C-delta Proto-Oncogene Proteins c-bcl-2 Caspases Reperfusion Injury bcl-Associated Death Protein Poly(ADP-ribose) Polymerases Carrier Proteins Proto-Oncogene Proteins c-akt Signal Transduction |
| Zdroj: | The Journal of biological chemistry. 279(46) |
| ISSN: | 0021-9258 |
| Popis: | Heart attacks caused by occlusion of coronary arteries are often treated by mechanical or enzymatic removal of the occlusion and reperfusion of the ischemic heart. It is now recognized that reperfusion per se contributes to myocardial damage, and there is a great interest in identifying the molecular basis of this damage. We recently showed that inhibiting protein kinase Cdelta (PKCdelta) protects the heart from ischemia and reperfusion-induced damage. Here, we demonstrate that PKCdelta activity and mitochondrial translocation at the onset of reperfusion mediates apoptosis by facilitating the accumulation and dephosphorylation of the pro-apoptotic BAD (Bcl-2-associated death promoter), dephosphorylation of Akt, cytochrome c release, PARP (poly(ADP-ribose) polymerase) cleavage, and DNA laddering. Our data suggest that PKCdelta activation has a critical proapoptotic role in cardiac responses following ischemia and reperfusion. |
| Databáze: | OpenAIRE |
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