Development of a SARS Coronavirus Vaccine from Recombinant Spike Protein Plus Delta Inulin Adjuvant
| Autor: | Clifton, McPherson, Richard, Chubet, Kathy, Holtz, Yoshikazu, Honda-Okubo, Dale, Barnard, Manon, Cox, Nikolai, Petrovsky |
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| Rok vydání: | 2016 |
| Předmět: |
SARS
viruses fungi Inulin virus diseases Manufacture Viral Vaccines Severe Acute Respiratory Syndrome Article body regions Coronavirus cGMP Adjuvants Immunologic Severe acute respiratory syndrome-related coronavirus MERS Delta inulin Spike Glycoprotein Coronavirus Animals Humans Baculovirus Vaccine Adjuvant Advax adjuvant |
| Zdroj: | Vaccine Design |
| ISSN: | 1940-6029 |
| Popis: | Given periodic outbreaks of fatal human infections caused by coronaviruses, development of an optimal coronavirus vaccine platform capable of rapid production is an ongoing priority. This chapter describes the use of an insect cell expression system for rapid production of a recombinant vaccine against severe acute respiratory syndrome coronavirus (SARS). Detailed methods are presented for expression, purification, and release testing of SARS recombinant spike protein antigen, followed by adjuvant formulation and animal testing. The methods herein described for rapid development of a highly protective SARS vaccine are equally suited to rapid development of vaccines against other fatal human coronavirus infections, e.g., the MERS coronavirus. |
| Databáze: | OpenAIRE |
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