Expression of phosphorylated Akt in oral carcinogenesis and its induction by nicotine and alkaline stimulation
| Autor: | Ho-Tai, Wu, Shun-Yao, Ko, Jenny Hwai-Jen, Fong, Kuo-Wei, Chang, Tsung-Yun, Liu, Shou-Yen, Kao |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Nicotine Dose-Response Relationship Drug Blotting Western Mouth Mucosa Epithelial Cells Hydrogen-Ion Concentration Immunohistochemistry Gene Expression Regulation Neoplastic Cell Transformation Neoplastic Case-Control Studies Cell Line Tumor Enzyme Induction Carcinoma Squamous Cell Humans Female Mouth Neoplasms Leukoplakia Oral Phosphorylation Precancerous Conditions Proto-Oncogene Proteins c-akt Cells Cultured Cell Line Transformed |
| Zdroj: | Journal of oral pathologymedicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology. 38(2) |
| ISSN: | 1600-0714 |
| Popis: | In Taiwan, it is well documented that cigarette smoking and areca nut chewing contribute to the risk of oral squamous cell carcinoma (OSCC). The role of phosphorylated Akt (p-Akt) in oral carcinogenesis induced by nicotine and alkaline environments was investigated.Immunohistochemistry (IHC) was used to detect p-Akt expression in cancerous (n = 30) precancerous (n = 30), and normal mucosa tissues (n = 10). Western blotting was used to detect time-dependent induction of p-Akt by 100 microM nicotine in normal human bronchial epithelial cell (NHBE), normal human oral keratinocytes (NHOK), immortalized human epithelial cells (HaCaT) and OEC-M1 cells, dose-dependent induction of p-Akt in OEC-M1 and HaCaT cells and pH effect of p-Akt in OEC-M1. The unpaired t-test and the Fisher's exact test were used to analyze the p-Akt immunoreactivity in various groups and its association with clinicopathological parameters.Higher p-Akt expression in cancerous group than in normal mucosa (P = 0.0002) and precancerous (P = 0.0049) groups was observed. A time-dependent increase in p-Akt in the NHBE, NHOK, HaCaT and OEC-M1 cell lines was observed with 100 microM nicotine treatment. The dose-dependent increase in p-Akt by nicotine treatment in HaCaT and OEC-M1 cells was obviously observed. Higher p-Akt expression in more alkaline environment (pH 8.0) was observed than at pH 7.4 in OEC-M1 cells.A potential role for increased p-Akt may relate to the dose and time of nicotine use. The potential role of an alkaline environment to enhance nicotine-related oral carcinogenesis may exist. |
| Databáze: | OpenAIRE |
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