| Autor: |
Xian-Guo, Xu, Ying, Liu, Shu, Chen, Xiao-Zhen, Hong, Su-Dan, Tao, Kai-Rong, Ma, Xiao-Fei, Lan, Ji, He, Fa-Ming, Zhu, Hang-Jun, Lyu |
| Rok vydání: |
2015 |
| Předmět: |
|
| Zdroj: |
Zhongguo shi yan xue ye xue za zhi. 23(5) |
| ISSN: |
1009-2137 |
| Popis: |
To detect platelet anti-HPA-1a and -1b antibodies using recombinant GPIIIa fragments coupled to Luminex beads.The sensitivity of 2 techniques, monoclonal antibody specific immobilization of platelet antigen (MAIPA) and Luminex bead assay, was compared using 12 twofold-serial dilutions (from neat to 1 in 2048) of an anti-HPA-1a WHO international standard. The specificity of Luminex assay to identify anti-HPA-1a and -1b antibodies was assessed using 8 negative or positive controls and 36 blinded samples provided by WHO Platelet Workshop.The sensitivity of MAIPA and Luminex bead assay to detect anti-HPA-1a was dilution 1/64 (i.e. 1.56 IU/ml) and far more than dilution 1/2048 (i.e. 0.049 IU/mL), respectively. The Luminex bead assay could specifically identify negative and positive controls of anti-HPA-1a and -1b. All results of 36 blinded samples by Luminex assay were accordant to reference results except one sample which contained high concentration antithetical antibody and resulted in false positive of anti-HPA-1b. Cross-reactivity was also not observed with the samples containing HLA, ABO or other platelet antibodies.The Luminex beads coupled with recombinant GPIIIa fragments can be used to detect HPA-1 system antibodies with sufficient sensitivity and specificity, that is suitable for the detection of platelet alloantibodies in clinical alloimmune thrombocytopenia. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
