| Autor: |
Anne-Hélène, Pillet, Olivier, Juffroy, Virginie, Mazard-Pasquier, Jean-Louis, Moreau, Franck, Gesbert, Patricia, Chastagner, Jean-Hervé, Colle, Jacques, Thèze, Thierry, Rose |
| Rok vydání: |
2008 |
| Předmět: |
|
| Zdroj: |
European cytokine network. 19(1) |
| ISSN: |
1952-4005 |
| Popis: |
Two types of functional interleukin-2 receptor (IL-2Ralpha/IL-2Rbeta/gammac and IL-2Rbeta/gammac) have already been characterized in humans. Here we describe a new form consisting of IL-2Rbeta/beta homodimers that assemble spontaneously in the absence of gammac. Co-transfection of COS-7 cells with constructs expressing IL-2Rbeta chains tagged with either HA or MYC sequences results in the formation of IL-2Rbeta:HA/IL-2Rbeta:MYC complexes detectable by coimmunoprecipitation. The formation of these IL-2Rbeta:HA/IL-2Rbeta:MYC dimers is also observed in the absence of IL-2. Moreover, in COS cells expressing chimeras of IL-2Rbeta fused to fluorescence reporters such as IL-2Rbeta:ECFP and IL-2Rbeta:EYFP, we also observed specific FRET at the surface of living cells, as expected for dimer formation. Transiently transfected COS-7 cells expressing IL-2Rbeta bind 125I-labeled IL-2 (homodimers, Kd = 1nM) as cells expressing both IL-2Rbeta and gammac chains (heterodimers, Kd = 1 nM). IL-2Rbeta/IL-2Rbeta could represent either a decoy receptor or a new form of IL-2R involved in signaling when gammac expression is low. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
