| Autor: |
Olivier, De Wever, An, Hendrix, Astrid, De Boeck, Frank, Eertmans, Wendy, Westbroek, Geert, Braems, Marc E, Bracke |
| Rok vydání: |
2013 |
| Předmět: |
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| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 1070 |
| ISSN: |
1940-6029 |
| Popis: |
Tumor invasion is the outcome of a complex interplay between cancer cells and the stromal environment and requires the infiltration of a dense, cross-linked meshwork of collagen type I extracellular matrix. We use a membrane-free single-cell and spheroid-based complementary model to study cancer invasion through native collagen type I matrices. Cell morphology is preserved during the assays allowing real-time monitoring of invasion-induced changes in cell structure and F-actin organization. Combination of these models with computerized quantification permits the calculation of highly reproducible and operator-independent data. These assays are versatile in the use of fluorescent probes and have a flexible kinetic endpoint. Once the optimal experimental conditions are empirically determined, the collagen type I invasion assays can be used for preclinical validation of small-molecule inhibitors targeting invasion. Initiation and monitoring of the single-cell and spheroid invasion model can be achieved in 8 h (over 3 days) and in 14 h (over 5 days), respectively. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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