Measurement of mitochondrial oxygen consumption rates in mouse primary neurons and astrocytes

Autor:	Sofia M, Ribeiro, Alfredo, Giménez-Cassina, Nika N, Danial
Rok vydání:	2014
Předmět:	Cerebral Cortex Neurons Mice Oxygen Consumption Astrocytes Cell Respiration Animals Microtechnology Extracellular Space Mitochondria
Zdroj:	Methods in molecular biology (Clifton, N.J.). 1241
ISSN:	1940-6029
Popis:	The introduction of microplate-based assays that measure extracellular fluxes in intact, living cells has revolutionized the field of cellular bioenergetics. Here, we describe a method for real time assessment of mitochondrial oxygen consumption rates in primary mouse cortical neurons and astrocytes. This method requires the Extracellular Flux Analyzer Instrument (XF24, Seahorse Biosciences), which uses fluorescent oxygen sensors in a microplate assay format.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::e475a91224b75d4da1e68882f33ad1c4 https://pubmed.ncbi.nlm.nih.gov/25308488 Zobrazit plný text záznamu