| Autor: |
Pierre, Jacob, Nak Hyun, Kim, Feihua, Wu, Farid, El-Kasmi, Yuan, Chi, William G, Walton, Oliver J, Furzer, Adam D, Lietzan, Sruthi, Sunil, Korina, Kempthorn, Matthew R, Redinbo, Zhen-Ming, Pei, Li, Wan, Jeffery L, Dangl |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Science |
| ISSN: |
1095-9203 |
| Popis: |
Plant nucleotide-binding leucine-rich repeat receptors (NLRs) regulate immunity and cell death. In Arabidopsis, a subfamily of “helper” NLRs is required by many “sensor” NLRs. Active NRG1.1 oligomerized, was enriched in plasma membrane puncta, and conferred cytoplasmic calcium ion (Ca(2+)) influx in plant and human cells. NRG1.1-dependent Ca(2+) influx and cell death were sensitive to Ca(2+) channel blockers and were suppressed by mutations affecting oligomerization or plasma membrane enrichment. Ca(2+) influx and cell death mediated by NRG1.1 and ACTIVATED DISEASE RESISTANCE 1 (ADR1), another helper NLR, required conserved negatively charged N-terminal residues. Whole-cell voltage-clamp recordings demonstrated that Arabidopsis helper NLRs form Ca(2+)-permeable cation channels to directly regulate cytoplasmic Ca(2+) levels and consequent cell death. Thus, helper NLRs transduce cell death signals directly. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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