| Autor: |
M F, Turchinskiĭ, S N, Shcherbo, V D, Knorre, T B, Kolesnik, Iu V, Aleĭnik |
| Rok vydání: |
1991 |
| Předmět: |
|
| Zdroj: |
Bioorganicheskaia khimiia. 17(6) |
| ISSN: |
0132-3423 |
| Popis: |
A new reagent, polyphotobiotin (PPhB), for labelling DNA probes has been obtained using low molecule oligoethylenimine (M 600 Da). Photoreactive group of PPhB is 4-azido-salicylic acid. The procedure is simple and quick, the reaction time being about 20 min. PPhB-labelled DNA has the same efficiency in the hybridisation analysis as DNA labelled with Bio-4-dUTP via PCR. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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