| Autor: |
Mitsuhiro, Suenaga, Akihiko, Yamaguchi, Hiroshi, Soda, Koji, Orihara, Yuichi, Tokito, Yoshimune, Sakaki, Megumi, Umehara, Kenji, Terashi, Nakaaki, Kawamata, Mikio, Oka, Shigeru, Kohno, Chuwa, Tei |
| Rok vydání: |
2006 |
| Předmět: |
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| Zdroj: |
Anticancer research. 26(5A) |
| ISSN: |
0250-7005 |
| Popis: |
Gefitinib (Iressa, ZD1839) is a selective epidermal growth factor receptor tyrosine kinase inhibitor. E2F-1 is a critical determinant in cell cycle. Growth signals up-regulate telomerase activity. The effects of gefitinib on E2F-1 and telomerase in A549, H23 and A431 cells were examined.Cell proliferation and cell cycle progression were measured by the WST-1 assay and flow cytometry. The expression of E2F-1 and cyclin-dependent kinase inhibitors was evaluated, and hTERT mRNA expression and telomerase activity were analyzed.In the A431 and A549 cells, treatment with gefitinib inhibited cell proliferation and was associated with an increase in G1-phase. In both cell types, gefitinib decreased the expression of E2F-1 mRNA and protein, followed by the suppression of hTERT mRNA and telomerase activity. In the H23 cells, gefitinib did not affect cell proliferation.The antiproliferative effects of gefitinib may be, at least in part, due to the inhibition of E2F-1 expression and telomerase activity. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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