Detection and diversity of viruses infecting African yam (

Autor: Yacouba, Bakayoko, Amani M, Kouakou, Abou B, Kouassi, Rose-Marie, Gomez, Konan E B, Dibi, Brice S, Essis, Boni, N'Zué, Patrick, Adebola, Assanvon S-P, N'Guetta, Marie, Umber
Rok vydání: 2020
Předmět:
Zdroj: Plant Pathology
ISSN: 0032-0862
Popis: Yam (Dioscorea spp.) is a major staple food whose production is hampered by viral diseases. However, the prevalence, diversity, transmission, and impact of yam‐infecting viruses remain poorly documented. This study reports on the symptomatology, prevalence, and molecular diversity of eight viruses in 38 D. rotundata accessions from a germplasm collection and 206 F1 hybrid progenies maintained in Côte d'Ivoire. Mean severity scores as assessed from leaf symptoms ranged from 2 to 4 in the germplasm collection and from 1 to 3 in F1 hybrids, respectively. Dioscorea mosaic‐associated virus (DMaV), potexviruses, and yam mosaic virus (YMV) were detected by PCR‐based diagnosis tools in single and mixed infections in both the D. rotundata collection and F1 progenies, whereas badnaviruses were detected only in the germplasm collection. In contrast, cucumber mosaic virus (CMV), yam macluraviruses, yam asymptomatic virus 1 (YaV1), and yam mild mosaic virus (YMMV) could not be detected. No correlation could be established between severity scores and indexing results. Phylogenetic analysis performed on partial viral sequences amplified from infected samples unveiled the presence of two putative novel viral species belonging to genera Badnavirus and Potexvirus and provided evidence for plant‐to‐plant transmission of YMV, DMaV, and yam potexviruses.
A survey of leaf symptoms and viral molecular indexation on African yam germplasm and two hybrid populations unveiled a high prevalence of yam viruses in Côte d'Ivoire and provided evidence for their plant‐to‐plant transmission. ​
Databáze: OpenAIRE
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