Cytotoxicity and endothelial cell adhesion of lyophilized and irradiated bovine pericardium modified with silk fibroin and chitosan
| Autor: | Andrea C D, Rodas, Roberta, Polak, Priscila H, Hara, Emily I, Lee, Ronaldo N M, Pitombo, Olga Z, Higa |
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| Rok vydání: | 2011 |
| Předmět: |
Bioprosthesis
Heart Valve Prosthesis Implantation Chitosan Cell Survival Endothelial Cells CHO Cells Tissue Culture Techniques Cricetulus Cross-Linking Reagents Freeze Drying Cricetinae Heart Valve Prosthesis Materials Testing Cell Adhesion Animals Humans Cattle Collagen Fibroins Pericardium Cell Proliferation |
| Zdroj: | Artificial organs. 35(5) |
| ISSN: | 1525-1594 |
| Popis: | Grafts of biological tissues have been used since the 1960s as an alternative to the mechanical heart prostheses. Nowadays, the most consolidated treatment to bovine pericardial (BP) bioprostheses is the crosslinking with glutaraldehyde (GA), although GA may induce calcification in vivo. In previous work, our group demonstrated that electron beam irradiation applied to lyophilized BP in the absence of oxygen promoted crosslinks among collagen fibers of BP tissue. In this work, the incorporation of silk fibroin (SF) and chitosan (CHIT) in the BP not treated with GA was studied. The samples were irradiated and then analyzed for their cytotoxicity and the ability of adhesion and growth of endothelial cells. Initially, all samples showed cytotoxicity. However, after a few washing cycles, the cytotoxicity due to acetic acid and ethanol residues was removed from the biomaterial making it suitable for the biofunctional test. The samples modified with SF/CHIT and electron beam irradiated favored the adhesion and growth of endothelial cells throughout the tissue. |
| Databáze: | OpenAIRE |
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