| Autor: |
S, el Antri, O, Mauffret, M, Monnot, E, Lescot, O, Convert, S, Fermandjian |
| Rok vydání: |
1993 |
| Předmět: |
|
| Zdroj: |
Journal of molecular biology. 230(2) |
| ISSN: |
0022-2836 |
| Popis: |
CpG sites in DNA are hotspots for mutations leading to human genetic disorders. However, the structural basis for these events were still unclear and necessitated a deeper evaluation. Our experiments with phosphorus-31 nuclear magnetic resonance, ultraviolet-melting and circular dichroism on two related CpG-containing octanucleotide duplexes show that CpG is a malleable step whose conformation and thermal stability are strongly dependent on the nature of its flanking steps. We conclude that the CpG step may exert a deleterious structural influence on the helix very much like the mismatch containing steps. This peculiar property of CpG should constitute a molecular basis for its recognition by various ligands as well as for mutations affecting CpG and hence an explanation for its rarity in vertebrate genomes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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