[A method of determining heparin in blood plasma based on its antithrombin activity]

Autor: R E, Taktashev, A V, Suvorova, V E, Miller, O I, Kolesnikova
Rok vydání: 1990
Předmět:
Zdroj: Laboratornoe delo. (12)
ISSN: 0023-6748
Popis: The suggested method for measuring blood plasma heparin is based on heparin ability to enhance antithrombin activity of antithrombin III (AT-III), the major Xa and thrombin inhibitor. The method consists in measurement of blood plasma AT-III activity in the presence and absence of protamine sulfate that destroys the heparin--AT-III complex. Heparin content in U/ml is determined from the difference in the activities of heparin--AT-III complex and AT-III proper activity represented on the calibration curve. The method is sufficiently sensitive, it permits registration of heparin concentrations in a wide band (from 0.01 U/ml to 0.75 U/ml of plasma).
Databáze: OpenAIRE