Immunological markers that correlate with protection immunity against tularemia infection
| Autor: | Victoria V, Firstova, Alexander N, Mokrievich, Vitalii M, Pavlov, Aleksey A, Gorbatov, Tatiana I, Kombarova, Sergey F, Biketov, Ivan A, Dyatlov |
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| Rok vydání: | 2014 |
| Předmět: |
Antigens
Bacterial Immunity Cellular Mice Inbred BALB C Tumor Necrosis Factor-alpha Interleukins T-Lymphocytes Gene Expression Vaccines Attenuated Toll-Like Receptor 2 Immunophenotyping Interferon-gamma Mice Antigens CD Bacterial Vaccines Animals Immunization Francisella tularensis Tularemia Biomarkers Cells Cultured Spleen |
| Zdroj: | Advances in experimental medicine and biology. 808 |
| ISSN: | 0065-2598 |
| Popis: | An efficient immune response to tularemia is dependent on a strong cell-mediated component. We tried to identify markers of cellular immune responses that indicate a vaccine efficacy against tularemia. BALB/c mice were immunized with mutant F. tularensis 15∆23A and/or F. tularensis 15 NIIEG strains and then were challenged i.n. with F. tularensis Schu. We compared the influence of F. tularensis antigens (tularinum) in vitro on production of IL-1, IL-5, IL-6, IL-17, IFN-γ, and TNF-α by splenocytes obtained from intact mice and mice immunized with mutant F. tularensis 15∆23A and/or F. tularensis 15 NIIEG strains. We also compared expression of CD28, CD154, TLR-2, and CD69 markers on CD4 and CD8 T-cells after activation with tularinum in vitro. We found that tularinum-induced CD4(+) T-cells increased TNF-α and IFN-γ synthesis and expression of CD69 only in group mice with high degree of post immunization protection against F. tularensis Schu challenge. Estimation of CD69 expression on CD3(+)CD4(+) cells and IFN-γ, TNF-α synthesis by CD4(+) T-lymphocytes could be useful for determination protect ability of antitularemia immunity. |
| Databáze: | OpenAIRE |
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