| Autor: |
R, Bravo, H, Macdonald-Bravo, R, Müller, D, Húbsch, J M, Almendral |
| Rok vydání: |
1987 |
| Předmět: |
|
| Zdroj: |
Experimental cell research. 170(1) |
| ISSN: |
0014-4827 |
| Popis: |
We have studied the effect of the potent mitogen bombesin on the expression of c-fos and c-myc genes in quiescent mouse fibroblasts. We have demonstrated that bombesin rapidly induces a transient expression of c-fos mRNA followed by a more protracted elevation in c-myc mRNA levels. The intensity of the induction of expression of both proto-oncogenes depended on the dose of bombesin used. Prolonged treatment of the cells with TPA, which causes a selective decrease in protein kinase C activity, partially inhibited the induction of c-fos and c-myc gene expression by bombesin, similar to what has been observed with PDGF. However, a dramatic inhibition of the mitogenic response to bombesin--but not to PDGF--was found in TPA-treated cells. In contrast, TPA-treated cells showed an increased response to EGF with regard to proto-oncogene expression. The role of protein kinase C and Ca2+-dependent pathways in proto-oncogene induction by bombesin is discussed. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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