Differentially regulated promoters for antigen expression in Salmonella vaccine strains

Autor:	Miloš, Stojanov, Hervé, Besançon, Tiia, Snäkä, Denise, Nardelli-Haefliger, Roy, Curtiss, David, Baud
Rok vydání:	2019
Předmět:	Salmonella typhimurium Mice Mice Inbred BALB C Vaccines Synthetic Bacterial Proteins Salmonella Vaccines Animals Promoter Regions Genetic Vaccines Attenuated Antibodies Bacterial
Zdroj:	Vaccine. 38(26)
ISSN:	1873-2518
Popis:	In most attenuated Salmonella enterica vaccines, heterologous antigens are expressed under the control of strong inducible promoters to ensure a high level of synthesis. Although high expression levels of the antigen can improve the immunogenicity of the vaccine, they might be toxic to the Salmonella carrier. Expression problems could be avoided by the use of promoters with specific characteristics with respect to strength and timing of expression. To study the expression of ten selected promoters, translational promoter-green fluorescent protein (GFP) fusions were analyzed in three attenuated Salmonella strains, Ty21a, SL3261 and PhoP
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::e289f6bad12a06b9ef794fbb2259db51 https://pubmed.ncbi.nlm.nih.gov/32376109 Zobrazit plný text záznamu Full Text from ScienceDirect