Effect of exogenous recombinant human granulocyte and granulocyte-macrophage colony-stimulating factor on neutrophil function following allogeneic bone marrow transplantation
| Autor: | I, Fabian, Y, Kletter, I, Bleiberg, M, Gadish, E, Naparsteck, S, Slavin |
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| Rok vydání: | 1991 |
| Předmět: |
Adult
Male Neutrophils Anemia Aplastic Granulocyte-Macrophage Colony-Stimulating Factor Precursor Cell Lymphoblastic Leukemia-Lymphoma Recombinant Proteins Leukemia Myeloid Acute Phagocytosis Superoxides Granulocyte Colony-Stimulating Factor Leukemia Myeloid Chronic-Phase Humans Transplantation Homologous Female Bone Marrow Transplantation |
| Zdroj: | Experimental hematology. 19(9) |
| ISSN: | 0301-472X |
| Popis: | Functional activity of peripheral blood granulocytes was assessed in seven patients and in their normal donors following allogeneic bone marrow transplantation (BMT). Functions studied included superoxide generation (O2-), intracellular killing of Staphylococcus aureus, phagocytosis, and killing of Candida albicans. Neutrophils were tested following preincubation with 300 pM granulocyte-macrophage colony-stimulating factor (GM-CSF), 1.2 nM granulocyte colony-stimulating factor (G-CSF), or buffered solution (diluent) as control. Our data indicate that following BMT, both recipients and their normal donors show GM-CSF- and G-CSF-induced increases in: 1) O2- production in response to fMet-Leu-Phe (fMLP), 2) killing of S. aureus, and 3) phagocytosis of C. albicans. In two patients that showed low candidacidal activity, GM-CSF and G-CSF markedly enhanced the cytotoxic activity of the cells. Our studies indicate that GM-CSF and G-CSF increase "oxygen-dependent" oxidative activities in neutrophils from BMT recipients and their normal donors and enhance the antimicrobial activity of the cells. |
| Databáze: | OpenAIRE |
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