[Isolation of protoplasts of Xanthomonas rubrilineans and their use in the study of localization of aminopeptidases]

Autor: G N, Telesnina, N A, Vasil'eva, I N, Krest'ianova, Iu E, Bartoshevich, Iu O, Sazykin
Rok vydání: 1992
Předmět:
Zdroj: Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic]. 37(4)
ISSN: 0235-2990
Popis: The culture of Xanthomonas rubrilineans was able to synthesize a number of intracellular aminopeptidases. To study localization of the enzymes in the cells, a protoplasting procedure was developed providing the yield of 99.7 per cent. The following subcellular fractions were isolated: periplasmic, cytoplasmic and membranous. It was shown that alanine aminopeptidase was a cytoplasmic enzyme and glutamate peptidase was a membrane-bound enzyme.
Databáze: OpenAIRE