Sequence‐specific DNA binding by AT‐hook motifs in MeCP2

Autor:	Lyst, Matthew J., Connelly, John, Merusi, Cara, Bird, Adrian
Jazyk:	angličtina
Rok vydání:	2016
Předmět:	congenital hereditary and neonatal diseases and abnormalities Molecular Basis of Disease Base Sequence Methyl-CpG-Binding Protein 2 Mutation Missense DNA Methylation Research Letters Chromatin DNA-Binding Proteins Cytosine Rett syndrome Research Letter AT‐hook Humans AT-Hook Motifs MeCP2
Zdroj:	Febs Letters
ISSN:	1873-3468 0014-5793
Popis:	MeCP2 is a chromatin-associated protein that is mutated in Rett syndrome. Its methyl-CpG-binding domain interacts with DNA containing methylated cytosine, but other modes of recruitment to the genome have also been proposed. Here, we use in vitro and in vivo assays to investigate the DNA binding specificity of two AT-hook motifs in MeCP2. One exhibits robust sequence-specific DNA binding, whereas the other is a much weaker AT-hook. Our data indicate that these motifs are secondary contributors to DNA binding by MeCP2, and this view is supported by the absence of disease-causing missense mutations at these sites.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::e1e18c766d3c59bd1c3fb96cbdad387d http://europepmc.org/articles/PMC5028900 Zobrazit plný text záznamu Plný text